Species-specific effects of iron on temperate and tropical marine rotifers in reproduction, lipid and ROS metabolisms

Two euryhaline rotifers, the temperate species Brachionus plicatilis and tropical rotundiformis , were used to investigate effects of iron (FeSO 4 ·7H 2 O), an essential trace metal, on reproductive patterns lifetables, including metabolism lipid reactive oxygen (ROS). B. was more sensitive with regard sexual reproduction. While had no significant effect population growth at 0–48 μg/mL, it caused a decrease in resting egg production. exposed 6 12 μg/mL showed increase intracellular ROS levels neutral content organs, accompanied by downregulation antioxidant components CuZnSOD two cytochromes ( CYP clan 2&3). These suggested that iron-induced oxidative stress not neutralized its defense system thus negatively affecting fecundity fertilized mictic females. However, dose-dependent extended lifespan positive reproduction response 0–24 iron. Furthermore, compared Fe-exposed better mechanism, whereas genes involved synthesis (citrate lyase, mitochondrial ) vasa sirtuin-2 significantly upregulated control, implying likely have higher resilience stress. findings suggest is cause interspecific interactions complex, may evolved effective mechanism against • Iron varied rotifers. affected species. stimulated should affect rotifer metabolism.